PF3D7_0308900 (SF3B1)

Genome location: Pf3D7_03_v3:378,896..383,721(-)

Genome classification: Core

Function and Localization

Product Description: splicing factor 3B subunit 1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005686	U2 snRNP
Component	GO:0071004	U2-type prespliceosome
Component	GO:0071013	catalytic step 2 spliceosome
Function	GO:0003723	RNA binding
Function	GO:0003729	mRNA binding
Process	GO:0000398	mRNA splicing, via spliceosome
Process	GO:0000245	spliceosomal complex assembly

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	1.20	0.49
Trophozoite	expressed	1.29	0.38
Schizont	expressed	0.38	0.78
Gametocyte	expressed	0.72	0.61

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0308900

Resistome Missense Mutations: A682T

Resistome Compounds with Missense Mutations: AN13762, MMV084864, MMV1454442

Resistome # Samples with Disruptive Mutations: 15 (15 missense, 15 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -2.922 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0407100)

Relative Growth Rate: 0.27 ± 0.47
Confidence: 2.89

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O77327

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101702

Most Similar Human Ortholog: O75533

TM-align score: 0.72 | RMSD: 4.52

Seq Identity: 0.57 | Length: 1031 / 1386

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000115524	splicing factor 3b subunit 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	11	57	42	84
disruptive	47	102	57	142
missense	34	93	51	118

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	19	143	63	80
disruptive	81	218	88	253
missense	54	185	78	187

PlasmoDB Total SNPs: 163

Non-coding: 61 | Synonymous: 53 | Nonsynonymous: 48 | Stop Codon: 1

Protein Information

Protein Length: 1386 | Molecular Weight (kDa): 160.046

UniProt ID(s): O77327

PDB ID(s): None

Isoelectric Point: 6.75

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR015016	Splicing factor 3B subunit 1
InterPro	IPR016024	Armadillo-type fold
InterPro	IPR038737	Splicing factor 3B subunit 1-like
PFam	PF08920	Splicing factor 3B subunit 1
Superfamily	SSF48371	Armadillo-type fold

PMID	Title	Authors	DOI/Link
10448855	The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum.	Bowman S, Lawson D, ..., Barrell BG	10.1038/22964
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104