PfTargetHuntHelper

Function and Localization

Product Description: 3'-5' exonuclease, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.11.1 (Exodeoxyribonuclease I);3.1.11.2 (Exodeoxyribonuclease III)

Curated GO (PlasmoDB):

Component

GO:0005739

mitochondrion

Expression by stage, third quartile (Malaria Cell Atlas): No data

More info:

Essentiality

Zhang Phenotype: Non - Mutable in CDS

Mutagenesis Index Score (MIS): 0.121
Mutagenesis Fitness Score (MFS): -3.001
Number of insertions in CDS: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0706500)

Relative Growth Rate: 0.12 ± 0.11
Confidence: 5.88

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IB75

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

3.1.11.1	exodeoxyribonuclease I	NTPEDTAcamptothecinendonuclease CtIPfullerene-oligonucleotide conjugatesingle-stranded DNA-binding protein5'-thiophosphorylated oligonucleotide
3.1.11.2	exodeoxyribonuclease III	DNAPNAEDTAZnCl2p-chloromercuribenzoate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_151531

No human ortholog(s)

Protein Information

Protein length: 406 | Molecular Weight (kDa): 48.101

UniProt ID(s): Q8IB75

PDB ID(s): None

Isoelectric Point: 8.98

Protein Domain Annotations:

InterPro	IPR002562	3'-5' exonuclease domain
InterPro	IPR012337	Ribonuclease H-like superfamily
PFam	PF01612	3'-5' exonuclease domain
Superfamily	SSF53098	Ribonuclease H-like superfamily

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0824100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	18	7	27
disruptive	18	60	33	89
missense	18	59	32	80

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	35	16	22
disruptive	32	140	49	120
missense	28	125	44	81

PlasmoDB Total SNPs: 62

Non-coding: 25 | Synonymous: 26 | Nonsynonymous: 11 | Stop Codon: 0

Associated Publications

PMID	Title	Authors	DOI
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21