Genomic location: Pf3D7_09_v3:566,578..568,830(+)
Miles Classification: Core
Product Description: 26S proteasome regulatory subunit RPN8, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: N/A
Curated GO (PlasmoDB):
| Component | GO:0020020 | food vacuole |
| Component | GO:0000502 | proteasome complex |
| Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
Expression by stage, third quartile (Malaria Cell Atlas): No data
More info:
Zhang Phenotype: Non - Mutable in CDS
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0813900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I323
"Best" AlphaFill ligand hit: ETE (2-{2-[2-2-(methoxy-ethoxy)-ethoxy]-ethoxy}-ethanol, Local RMSD=1.27) with 2O95 (Global RMSD=2.82)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102054
Most Similar Human Ortholog: H3BNT7
All Human Orthologs (OrthoMCL):
| ENSG00000103035 | proteasome 26S subunit, non-ATPase 7 |
Protein length: 338 | Molecular Weight (kDa): 39.136
UniProt ID(s): Q8I323
PDB ID(s): None
Isoelectric Point: 8.27
Protein Domain Annotations:
| InterPro | IPR000555 | JAB1/MPN/MOV34 metalloenzyme domain |
| InterPro | IPR024969 | Rpn11/EIF3F, C-terminal |
| InterPro | IPR033858 | 26S Proteasome non-ATPase regulatory subunit 7/8 |
| PFam | PF01398 | JAB1/MPN/MOV34 metalloenzyme domain |
| PFam | PF13012 | Rpn11/EIF3F, C-terminal |
| Superfamily | N/A | N/A |
Old (Pf3D7v3) Gene ID: PF3D7_0912900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 14 | 14 | 27 |
| disruptive | 9 | 15 | 14 | 24 |
| missense | 4 | 9 | 6 | 20 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 44 | 22 | 18 |
| disruptive | 16 | 42 | 28 | 65 |
| missense | 5 | 23 | 19 | 37 |
PlasmoDB Total SNPs: 158
Non-coding: 123 | Synonymous: 27 | Nonsynonymous: 7 | Stop Codon: 1
| PMID | Title | Authors | DOI |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 22927375 | Near-atomic resolution structural model of the yeast 26S proteasome | Beck F, Unverdorben P, ..., Forster F | 10.1073/pnas.1213333109 |
| 23271117 | The degradation of kinesin-like calmodulin binding protein of D. salina (DsKCBP)is mediated by the ubiquitin-proteasome system | Shi K, Li J, ..., Xue L | 10.1007/s11033-012-2385-2 |
| 28844860 | An AAA Motor-Driven Mechanical Switch in Rpn11 Controls Deubiquitination at the26S Proteasome | Worden EJ, Dong KC, Martin A | 10.1016/j.molcel.2017.07.023 |
| 32763142 | Tightening the Crosslinking Distance Restraints for Better Resolution of ProteinStructure and Dynamics | Gong Z, Ye SX, Tang C | 10.1016/j.str.2020.07.010 |
| 17559875 | The crystal structure of the human Mov34 MPN domain reveals a metal-free dimer | Sanches M, Alves BS, Zanchin NI, Guimaraes BG | 10.1016/j.jmb.2007.04.084 |
| 15221960 | Post-translationally modified S12, absent in transformed breast epithelial cells,is not associated with the 26S proteasome and is induced by proteasome inhibitor | Thompson HG, Harris JW, Brody JP | 10.1002/ijc.20261 |
| 34906219 | A cis-regulatory-directed pipeline for the identification of genes involved incardiac development and disease | Nim HT, Dang L, ..., Ramialison M | 10.1186/s13059-021-02539-0 |
| 24463465 | Structure of the Rpn11-Rpn8 dimer reveals mechanisms of substratedeubiquitination during proteasomal degradation | Worden EJ, Padovani C, Martin A | 10.1038/nsmb.2771 |
| 22474342 | Similar temporal and spatial recruitment of native 19S and 20S proteasomesubunits to transcriptionally active chromatin | Geng F, Tansey WP | 10.1073/pnas.1200854109 |
| 15018611 | Participation of the proteasomal lid subunit Rpn11 in mitochondrial morphologyand function is mapped to a distinct C-terminal domain | Rinaldi T, Pick E, ..., Glickman MH | 10.1042/BJ20040008 |
| 27053109 | The Proteasome Subunit Rpn8 Interacts with the Small Nucleolar RNA Protein(snoRNP) Assembly Protein Pih1 and Mediates Its Ubiquitin-independent Degradationin Saccharomyces cerevisiae | Paci A, Liu PX, Zhang L, Zhao R | 10.1074/jbc.M115.702043 |
| 26182356 | Base-CP proteasome can serve as a platform for stepwise lid formation | Yu Z, Livnat-Levanon N, ..., Glickman MH | 10.1042/BSR20140173 |
| 24516147 | Crystal structure of the proteasomal deubiquitylation module Rpn8-Rpn11 | Pathare GR, Nagy I, ..., Baumeister W | 10.1073/pnas.1400546111 |
| 35439336 | Silencing suppressors of rice black-streaked dwarf virus and rice stripe virushijack the 26S proteasome of Laodelphax striatellus to facilitate virusaccumulation and transmission | Li Y, Zhu L, ..., Zhu C | 10.1002/ps.6918 |
| 36060813 | Proteasomal subunit depletions differentially affect germline integrity in C.elegans | Fernando LM, Quesada-Candela C, ..., Allen AK | 10.3389/fcell.2022.901320 |
| 15735686 | Growth suppression induced by the TRC8 hereditary kidney cancer gene is dependentupon JAB1/CSN5 | Gemmill RM, Lee JP, ..., Drabkin HA | 10.1038/sj.onc.1208509 |
| 36350544 | High-Throughput Assay for Characterizing Rpn11 Deubiquitinase Activity | Xie G, Dong KC, Worden EJ, Martin A | 10.1007/978-1-0716-2803-4_6 |
| 30356695 | Computational Studies on the Inhibitor Selectivity of Human JAMMDeubiquitinylases Rpn11 and CSN5 | Kumar V, Naumann M, Stein M | 10.3389/fchem.2018.00480 |
| 25631053 | Solution structure of yeast Rpn9: insights into proteasome lid assembly | Hu Y, Wu Y, ..., Jin C | 10.1074/jbc.M114.626762 |
| 22307589 | Molecular architecture of the 26S proteasome holocomplex determined by anintegrative approach | Lasker K, Forster F, ..., Baumeister W | 10.1073/pnas.1120559109 |