Genomic location: Pf3D7_11_v3:1,731,176..1,734,347(-)
Miles Classification: Core
Product Description: DNA-directed RNA polymerases I and III subunit RPAC1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Component | GO:0005736 | RNA polymerase I complex |
| Component | GO:0005666 | RNA polymerase III complex |
| Process | GO:0042797 | tRNA transcription by RNA polymerase III |
| Process | GO:0006360 | transcription by RNA polymerase I |
Expression by stage, third quartile (Malaria Cell Atlas):
Ring: 1.99 | Trophozoite: 1.71 | Schizont: 0 | Gametocyte: 1.46
More info:
Zhang Phenotype: Non - Mutable in CDS
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IHT3
"Best" AlphaFill ligand hit: F3S (fe3-s4 cluster, Local RMSD=0.96) with 2PA8 (Global RMSD=7.58)
BRENDA EC Inhibitors:
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102180
Most Similar Human Ortholog: O15160
All Human Orthologs (OrthoMCL):
| ENSG00000171453 | RNA polymerase I and III subunit C |
Protein length: 333 | Molecular Weight (kDa): 38.963
UniProt ID(s): Q8IHT3
PDB ID(s): None
Isoelectric Point: 7.23
Protein Domain Annotations:
| InterPro | IPR011262 | DNA-directed RNA polymerase, insert domain |
| InterPro | IPR011263 | DNA-directed RNA polymerase, RpoA/D/Rpb3-type |
| InterPro | IPR033901 | DNA-directed RNA polymerases I and III subunit AC40 |
| InterPro | IPR036603 | RNA polymerase, RBP11-like subunit |
| InterPro | IPR036643 | DNA-directed RNA polymerase, insert domain superfamily |
| PFam | PF01000 | DNA-directed RNA polymerase, insert domain |
| PFam | PF01193 | DNA-directed RNA polymerase, RpoA/D/Rpb3-type |
| Superfamily | SSF55257 | RNA polymerase, RBP11-like subunit |
| Superfamily | SSF56553 | DNA-directed RNA polymerase, insert domain superfamily |
Old (Pf3D7v3) Gene ID: PF3D7_1143300
Resistome Missense Mutations: Q95E
Resistome Compounds with Missense Mutations: SJ733
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 103
Non-coding: 83 | Synonymous: 12 | Nonsynonymous: 7 | Stop Codon: 1
| PMID | Title | Authors | DOI |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 2203770 | ABF1 binding sites in yeast RNA polymerase genes | Della Seta F, Treich I, Buhler JM, Sentenac A | |
| 7929437 | High conservation of subunit composition of RNA polymerase I(A) between yeast andmouse and the molecular cloning of mouse RNA polymerase I 40-kDa subunit RPA40 | Song CZ, Hanada K, ..., Muramatsu M | |
| 2830265 | RPA190, the gene coding for the largest subunit of yeast RNA polymerase A | Memet S, Gouy M, ..., Buhler JM | |
| 10079952 | [Molecular cloning and characteristics of rpc19+ and rpc40+ Schizosaccharomycespombe genes, coding for common subunits of nuclear RNA polymerase I and III] | Shpakovskii GV, Shematorova EK | |
| 16730080 | Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei | Nguyen TN, Schimanski B, ..., Gunzl A | |
| 34055890 | Specific Features of RNA Polymerases I and III: Structure and Assembly | Turowski TW, Boguta M | 10.3389/fmolb.2021.680090 |
| 3815519 | RPC40, a unique gene for a subunit shared between yeast RNA polymerases A and C | Mann C, Buhler JM, Treich I, Sentenac A | 10.1016/0092-8674(87)90241-8 |
| 12860698 | Study of biological characteristics of spotted fever group rickettsial genotypesRpA4, DnS14, and DnS28 | Samoilenko IE, Rudakov NV, ..., Kumpan LV | 10.1111/j.1749-6632.2003.tb07435.x |
| 8164651 | Suppression of yeast RNA polymerase III mutations by FHL1, a gene coding for afork head protein involved in rRNA processing | Hermann-Le Denmat S, Werner M, Sentenac A, Thuriaux P | 10.1128/mcb.14.5.2905-2913.1994 |
| 2112240 | A conjugation-specific gene (cnjC) from Tetrahymena encodes a protein homologousto yeast RNA polymerase subunits (RPB3, RPC40) and similar to a portion of theprokaryotic RNA polymerase alpha subunit (rpoA) | Martindale DW | 10.1093/nar/18.10.2953 |
| 10541858 | Rpc19 and Rpc40, two alpha-like subunits shared by nuclear RNA polymerases I andIII, are interchangeable between the fission and budding yeasts | Shpakovski GV, Shematorova EK | 10.1007/s002940050492 |
| 25931562 | Retrotransposons. An RNA polymerase III subunit determines sites ofretrotransposon integration | Bridier-Nahmias A, Tchalikian-Cosson A, ..., Lesage P | 10.1126/science.1259114 |
| 10628857 | Isolation and characterization of the fission yeast gene rpa42+, which encodes asubunit shared by RNA polymerases I and III | Imazawa Y, Imai K, ..., Nogi Y | 10.1007/pl00013818 |
| 8516295 | Interactions between three common subunits of yeast RNA polymerases I and III | Lalo D, Carles C, Sentenac A, Thuriaux P | 10.1073/pnas.90.12.5524 |
| 9540830 | Cloning and characterization of the human RNA polymerase I subunit hRPA40 | Dammann R, Pfeifer GP | 10.1016/s0167-4781(97)00206-6 |