Genomic location: Pf3D7_12_v3:644,215..646,624(+)
Miles Classification: Core
Product Description: serine--tRNA ligase, putative
SignalP Peptide: MHLSKIIFLLIILFPYHVNS
# Transmembrane Domains: 0
EC Numbers: 6.1.1.11 (Serine--tRNA ligase)
Curated GO (PlasmoDB):
| Component | GO:0020011 | apicoplast |
| Component | GO:0005829 | cytosol |
| Component | GO:0016020 | membrane |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004828 | serine-tRNA ligase activity |
| Process | GO:0006434 | seryl-tRNA aminoacylation |
Expression by stage, third quartile (Malaria Cell Atlas): No data
More info:
Zhang Phenotype: Non - Mutable in CDS
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1431700)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I5P7
"Best" AlphaFill ligand hit: SSA (5'-o-(n-(l-seryl)-sulfamoyl)adenosine, Local RMSD=0.30) with 3QO8 (Global RMSD=2.29)
BRENDA EC Inhibitors:
| 6.1.1.11 | serine-tRNA ligase | GSSGsiRNAglycinealanine3'-dATPcysteinethreonineSB-217452serinamidediphosphatetRNASer-C-CPoly-arginine... |
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_100275
Most Similar Human Ortholog: M0R259
All Human Orthologs (OrthoMCL):
| ENSG00000031698 | seryl-tRNA synthetase 1 |
| ENSG00000104835 | seryl-tRNA synthetase 2, mitochondrial |
| ENSG00000269547 | novel protein |
| ENSG00000282835 | novel protein |
| ENSG00000283104 | seryl-tRNA synthetase 2, mitochondrial |
Protein length: 618 | Molecular Weight (kDa): 73.272
UniProt ID(s): A0A143ZZQ0, A0A5J6C745, A0A5J6C7C8, A0A5J6C7K3, A0A5J6C856, A0A5J6C8P1, A0A5J6C8Q4, A0A5J6C949, A0A5J6C9Z3, Q8I5P7
PDB ID(s): None
Isoelectric Point: 10.14
Protein Domain Annotations:
| InterPro | IPR002314 | Aminoacyl-tRNA synthetase, class II (G/ P/ S/T) |
| InterPro | IPR002317 | Serine-tRNA ligase, type1 |
| PFam | PF00587 | Aminoacyl-tRNA synthetase, class II (G/ P/ S/T) |
| Superfamily | SSF55681 | N/A |
Old (Pf3D7v3) Gene ID: PF3D7_1216000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 25 | 15 | 42 |
| disruptive | 14 | 56 | 34 | 85 |
| missense | 14 | 55 | 34 | 77 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 50 | 33 | 45 |
| disruptive | 25 | 137 | 59 | 175 |
| missense | 17 | 122 | 51 | 126 |
PlasmoDB Total SNPs: 107
Non-coding: 55 | Synonymous: 34 | Nonsynonymous: 18 | Stop Codon: 0
| PMID | Title | Authors | DOI |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |