Genomic location: Pf3D7_13_v3:2,621,160..2,624,082(+)
Miles Classification: Core
Product Description: DNA topoisomerase 6 subunit B
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: N/A
Curated GO (PlasmoDB):
| Component | GO:0005739 | mitochondrion |
| Component | GO:0015935 | small ribosomal subunit |
| Function | GO:0003916 | DNA topoisomerase activity |
| Function | GO:0003824 | catalytic activity |
| Function | GO:0042802 | identical protein binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage, third quartile (Malaria Cell Atlas): No data
More info:
Zhang Phenotype: Mutable in CDS
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1141400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1141400)
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ID53
"Best" AlphaFill ligand hit: ALF (tetrafluoroaluminate ion, Local RMSD=1.24) with 1Z5B (Global RMSD=10.69)
No associated EC numbersNo evidence of orthology to BindingDB entries
Protein length: 561 | Molecular Weight (kDa): 66.083
UniProt ID(s): Q8ID53
PDB ID(s): None
Isoelectric Point: 4.8
Protein Domain Annotations:
| InterPro | IPR003594 | Histidine kinase/HSP90-like ATPase |
| InterPro | IPR036890 | Histidine kinase/HSP90-like ATPase superfamily |
| PFam | PF02518 | Histidine kinase/HSP90-like ATPase |
| Superfamily | SSF55874 | Histidine kinase/HSP90-like ATPase superfamily |
Old (Pf3D7v3) Gene ID: PF3D7_1365600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 35 | 20 | 42 |
| disruptive | 22 | 99 | 62 | 115 |
| missense | 15 | 84 | 58 | 104 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 64 | 34 | 49 |
| disruptive | 43 | 183 | 89 | 128 |
| missense | 26 | 166 | 81 | 94 |
PlasmoDB Total SNPs: 203
Non-coding: 141 | Synonymous: 39 | Nonsynonymous: 22 | Stop Codon: 1
| PMID | Title | Authors | DOI |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 27303712 | Radicicol-Mediated Inhibition of Topoisomerase V IB-VIA Activity of the Human Malaria Parasite Plasmodium falciparum | Chalapareddy S, Chakrabarty S, Bhattacharyya MK, Bhattacharyya S | 10.1128/mSphere.00025-15 |
| 17951446 | MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNAendoreduplication, chromatin condensation, and transcriptional silencing | Kirik V, Schrader A, Uhrig JF, Hulskamp M | 10.1105/tpc.107.054361 |
| 25482814 | A genetic screen for mutants defective in IAA1-LUC degradation in Arabidopsisthaliana reveals an important requirement for TOPOISOMERASE6B in auxinphysiology | Gilkerson J, Callis J | 10.4161/psb.29850 |
| 12119417 | A crucial role for the putative Arabidopsis topoisomerase VI in plant growth anddevelopment | Yin Y, Cheong H, ..., Chory J | 10.1073/pnas.152337599 |
| 29595473 | Topoisomerase VI senses and exploits both DNA crossings and bends to facilitatestrand passage | Wendorff TJ, Berger JM | 10.7554/eLife.31724 |
| 33398171 | Structural and functional characterization of the Spo11 core complex | Claeys Bouuaert C, Tischfield SE, ..., Keeney S | 10.1038/s41594-020-00534-w |
| 17921483 | Protist homologs of the meiotic Spo11 gene and topoisomerase VI reveal anevolutionary history of gene duplication and lineage-specific loss | Malik SB, Ramesh MA, Hulstrand AM, Logsdon JM Jr | 10.1093/molbev/msm217 |
| 36660855 | Cold-induced inhibition of photosynthesis-related genes integrated by a TOP6complex in rice mesophyll cells | Xu Z, Zhang J, ..., Chen W | 10.1093/nar/gkac1275 |
| 18759987 | Transcriptional responses to biologically relevant doses of UV-B radiation in themodel archaeon, Halobacterium sp. NRC-1 | Boubriak I, Ng WL, ..., McCready SJ | 10.1186/1746-1448-4-13 |