PfTargetHuntHelper

Function and Localization

Product Description: pre-mRNA-splicing factor CWF18, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: N/A

Curated GO (PlasmoDB):

Component	GO:0005684	U2-type spliceosomal complex
Component	GO:0005634	nucleus
Component	GO:0071014	post-mRNA release spliceosomal complex

Expression by stage, third quartile (Malaria Cell Atlas): No data

More info:

Essentiality

Zhang Phenotype: Mutable in CDS

Mutagenesis Index Score (MIS): 0.554
Mutagenesis Fitness Score (MFS): -2.03
Number of insertions in CDS: 1

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1315200)

Relative Growth Rate: 0.09 ± 0.03
Confidence: 8.14

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKW1

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103244

Most Similar Human Ortholog: S4R331

TM-align score: 0.34541
TM-align length: 40 / 147
TM-align RMSD: 3.07
TM-align sequence identity: 0.075

All Human Orthologs (OrthoMCL):

ENSG00000160799

coiled-coil domain containing 12

Protein Information

Protein length: 147 | Molecular Weight (kDa): 17.257

UniProt ID(s): Q8IKW1

PDB ID(s): None

Isoelectric Point: 4.74

Protein Domain Annotations:

InterPro	IPR013169	mRNA splicing factor Cwf18-like
PFam	PF08315	mRNA splicing factor Cwf18-like
Superfamily	N/A	N/A

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1451500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	0	0	0
disruptive	0	1	0	0
missense	0	0	0	0

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	0	0	0	0
disruptive	0	1	0	0
missense	0	0	0	0

PlasmoDB Total SNPs: 92

Non-coding: 80 | Synonymous: 10 | Nonsynonymous: 2 | Stop Codon: 0

Associated Publications

PMID	Title	Authors	DOI
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108